We have created two different inner ear pertinent cDNA microarrays:
Human Cochlea
A Human Cochlear cDNA Microarray is derived from clones from the Morton Fetal Cochlea cDNA Library. The array is comprised of over 5,000 individual cDNA clones, each PCR amplified, purified, and spotted twice. Additional deafness genes, as well as positive and negative controls, were added to the array. A list of the genes/ESTs found in the array can be found at right in Excel spreadsheet format (Zipped file).
Mouse Organ of Corti
A Mouse organ of Corti cDNA Microarray is derived from clones from a mouse organ of Corti cDNA library developed in the Kachar laboratory at NIH. More information regarding the library construction can be found herein Pompeiia et al. This cDNA array is comprised of over 9,000 individual cDNA clones, each PCR amplified, purified, and spotted once. Additional deafness genes, as well as positive and negative controls, were added to the array. A list of the genes/ESTs found in the array can be found at right in Excel spreadsheet format (Zipped file).
Obtaining cDNA Array Slides
We hope to be able to share these cDNA arrays with other investigators, either on a collaborative basis or by providing the arrays directly to investigators who have experience using microarrays. For inquiries, please contact Cynthia Morton at cmorton@partners.org or Anne Giersch at agiersch@rics.bwh.harvard.edu or fill out and fax the Request form at right.
Send Feedback to: 
