Human cochleas collected in the Pathology Department at Brigham and Women’s Hospital were used to make RNA and a unique cDNA library. The library was donated to the IMAGE Consortium for creation of Expressed Sequence Tags (ESTs) (
dbEST Library ID.371). Over 15,000 ESTs were deposited in GenBank. All clones are commercially available.
Morton Human Fetal Cochlea cDNA Library EST Data:
The link at right provides a zipped file of the complete collection of EST derived from the Morton Human Fetal cDNA Library. This is a large table! The table provides the GenBank Accession number of the ESTs and the identity of the corresponding gene (if known). There are slightly over 15,000 ESTs listed. If you do not have Winzip, you can download the application at: http://www.winzip.com/
Library Data:
The Morton Human Fetal cDNA library was cloned unidirectionally, primed with oligo dT, NOT subtracted or normalized. Starting material was human fetal cochlear mRNA, 16 – 22 weeks, phenotypically normal.
Cloning vector was lambda derivative, Uni-ZAP XR.
5' adaptor sequence: 5'-GAATTCGGCACGAG-3'
3' adaptor sequence: 5'-CTCGAGTTTTTTTTTTTTTTTTTT-3'
Plasmid vector is BlueScript SK-
Approximately 37% of inserts <0.5 kb, 56% 0.5-1.0 kb, 7% >1 kb.
Reference for cDNA library construction: Robertson et al. Genomics 23, 42-50 (1994)
Subtracted Human Cochlear Library EST Data:
A smaller cDNA library derived from human fetal mRNA collected in the Morton laboratory at Brigham and Women’s Hospital was constructed in the J. Battey lab at NIH (dbEST Library ID 1822 ).For creationof this library, human fetal cochlea cDNA was normalized to brain, testis, kidney, uterus, and fibroblast according to Clontech PCR-Select cDNA Subtraction method. There are 3880 ESTs listed.
The EST gene identification data was last updated in June 2006
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