Research SummaryMy research is focused on wound healing via gene therapy and healing environment changes. I collaborated with the Department of Dermatology, Children’s Hospital Boston to study the effects of adhesion molecules and growth factors on wound healing. Gene Therapy and Wound Healing 1. Gene transfer is an attractive therapeutic modality for the near future. Microseeding is currently the most efficient mechanical technique for in vivo gene transfer. I standardized and developed several new applications of the technique for internal organs, skin, and bone. I also demonstrated that the delivery of hBMP-2 (human bone morphogenetic protein) to the periosteum results in a greater amount of bone formation. Further research is underway. 2. Wound healing is triggered by multiple signals (cytokines, growth factors) released from stimulated cells. Genetic manipulation of the cells both in vivo and in vitro can accelerate wound healing. In our experiment with skin cell genetic manipulation, we found an increased production of growth factors and therefore a significant acceleration in wound healing. We also developed an unique method for skin cell harvesting and transplantation. 3. Gene therapy safety is dependent on the precise control of gene expression. Our laboratory developed a novel gene expression regulating switch, which is currently commercially available as T-RExTM from InvitrogenTM. I demonstrated in the circulating blood of mice that levels of gene expression can be precisely controlled. Further research is underway. Wound Healing Environment Skin regeneration is the most critical part of burn patients' recovery. By manipulating the skin wound environment, we can ensure that patients experience faster and better healing. In particular, I helped develop a wound chamber (currently being tested for marked use) that allows wounds to heal in a liquid environment. The liquid environment enables faster wound healing than any other treatment modality. Back to the top | 
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